bap1 expression pattern Search Results


bap1 expression pattern  (Human Protein Atlas)
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Human Protein Atlas bap1 expression pattern
<t>BAP1</t> depletion promotes MSC migration. (A) A table of the siRNA screening results. (B-G) The MSCs were transfected with 20 nM siNon or deubiquitinase siRNAs. The cells were scratched, visualized by a bright field microscope (E), and further analyzed using Image the J software (B, C). Data are presented as the mean ± standard deviation (SD) from three independent experiments. Statistical analysis was performed using two-way ANOVA. Significances were computed by Tukey’s honestly significant difference (HSD) (*P < 0.05, **P < 0.01, and ***P < 0.001). (D) BAP1 protein levels were determined by immunoblotting. For the transwell migration (F) and invasion analysis (G), data are presented as the mean ± SD from three independent experiments. Statistical analysis was performed using Student’s t-test (**P < 0.01 and ***P < 0.001).
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BAP1 depletion promotes MSC migration. (A) A table of the siRNA screening results. (B-G) The MSCs were transfected with 20 nM siNon or deubiquitinase siRNAs. The cells were scratched, visualized by a bright field microscope (E), and further analyzed using Image the J software (B, C). Data are presented as the mean ± standard deviation (SD) from three independent experiments. Statistical analysis was performed using two-way ANOVA. Significances were computed by Tukey’s honestly significant difference (HSD) (*P < 0.05, **P < 0.01, and ***P < 0.001). (D) BAP1 protein levels were determined by immunoblotting. For the transwell migration (F) and invasion analysis (G), data are presented as the mean ± SD from three independent experiments. Statistical analysis was performed using Student’s t-test (**P < 0.01 and ***P < 0.001).

Journal: BMB Reports

Article Title: BAP1 controls mesenchymal stem cell migration by inhibiting the ERK signaling pathway

doi: 10.5483/BMBRep.2023-0174

Figure Lengend Snippet: BAP1 depletion promotes MSC migration. (A) A table of the siRNA screening results. (B-G) The MSCs were transfected with 20 nM siNon or deubiquitinase siRNAs. The cells were scratched, visualized by a bright field microscope (E), and further analyzed using Image the J software (B, C). Data are presented as the mean ± standard deviation (SD) from three independent experiments. Statistical analysis was performed using two-way ANOVA. Significances were computed by Tukey’s honestly significant difference (HSD) (*P < 0.05, **P < 0.01, and ***P < 0.001). (D) BAP1 protein levels were determined by immunoblotting. For the transwell migration (F) and invasion analysis (G), data are presented as the mean ± SD from three independent experiments. Statistical analysis was performed using Student’s t-test (**P < 0.01 and ***P < 0.001).

Article Snippet: Furthermore, the BAP1 expression pattern observed in the Human Protein Atlas supports an inverse correlation between BAP1 expression and cell motility, with blood cells, known for their high motility, showing notably lower levels of BAP1 expression ( of the SI).

Techniques: Migration, Transfection, Microscopy, Software, Standard Deviation, Western Blot

BAP1 expression hinders MSC migration. (A) MSCs, 24 h post-pCGfd-GFP transfection, were visualized using a fluorescence microscope. (B) MSCs transfected with pCGfd-GFP or -HA-BAP1 were harvested for immunoblot analysis. (C, D) Cells were scratched, visualized by a bright field microscope, and further analyzed using the Image J software. Data are presented as the mean ± SD from three independent experiments. Statistical analysis was performed using two-way ANOVA. Significance was calculated using Tukey’s HSD (***P < 0.001). (E, F) Transwell migration or invasion assays were performed. Data are presented as the mean ± SD from three independent experiments. Statistical analysis was performed using a Student’s t-test (*P < 0.05 and **P < 0.01).

Journal: BMB Reports

Article Title: BAP1 controls mesenchymal stem cell migration by inhibiting the ERK signaling pathway

doi: 10.5483/BMBRep.2023-0174

Figure Lengend Snippet: BAP1 expression hinders MSC migration. (A) MSCs, 24 h post-pCGfd-GFP transfection, were visualized using a fluorescence microscope. (B) MSCs transfected with pCGfd-GFP or -HA-BAP1 were harvested for immunoblot analysis. (C, D) Cells were scratched, visualized by a bright field microscope, and further analyzed using the Image J software. Data are presented as the mean ± SD from three independent experiments. Statistical analysis was performed using two-way ANOVA. Significance was calculated using Tukey’s HSD (***P < 0.001). (E, F) Transwell migration or invasion assays were performed. Data are presented as the mean ± SD from three independent experiments. Statistical analysis was performed using a Student’s t-test (*P < 0.05 and **P < 0.01).

Article Snippet: Furthermore, the BAP1 expression pattern observed in the Human Protein Atlas supports an inverse correlation between BAP1 expression and cell motility, with blood cells, known for their high motility, showing notably lower levels of BAP1 expression ( of the SI).

Techniques: Expressing, Migration, Transfection, Fluorescence, Microscopy, Western Blot, Software

BAP1 suppresses the ERK signaling pathway. (A, B) MSCs transfected with 20 nM siNon or siBAP1 were harvested for immunoblot analysis. (C) The levels of mRNA were determined using qRT-PCR. Data are presented as the mean ± SD from three independent experiments. Statistical analysis was performed using Student’s t-test (ns = non-significant, *P < 0.05 and **P < 0.01).

Journal: BMB Reports

Article Title: BAP1 controls mesenchymal stem cell migration by inhibiting the ERK signaling pathway

doi: 10.5483/BMBRep.2023-0174

Figure Lengend Snippet: BAP1 suppresses the ERK signaling pathway. (A, B) MSCs transfected with 20 nM siNon or siBAP1 were harvested for immunoblot analysis. (C) The levels of mRNA were determined using qRT-PCR. Data are presented as the mean ± SD from three independent experiments. Statistical analysis was performed using Student’s t-test (ns = non-significant, *P < 0.05 and **P < 0.01).

Article Snippet: Furthermore, the BAP1 expression pattern observed in the Human Protein Atlas supports an inverse correlation between BAP1 expression and cell motility, with blood cells, known for their high motility, showing notably lower levels of BAP1 expression ( of the SI).

Techniques: Transfection, Western Blot, Quantitative RT-PCR

BAP1 binds to ERK1/2 and regulates its ubiquitination status in a deubiquitinase activity-dependent manner. (A, C, E) 293T cells transfected with the indicated plasmids were harvested, lysed, and immunoprecipitated with an anti-GFP antibody. Thereafter, proteins were analyzed by immunoblotting using anti-FLAG and -GFP antibodies. (B) MSCs were lysed and immunoprecipitated using an anti-BAP1 antibody. Endogenous proteins were analyzed by immunoblotting using the indicated antibodies. (D, F) 293T cells transfected with the indicated plasmids were harvested, lysed under denaturing conditions, and immunoprecipitated with an anti-GFP antibody. Protein levels were analyzed by immunoblotting using anti-ubiquitin-HRP, -GFP, and -FLAG antibodies. (G) MSCs transfected with 20 nM siNon or siBAP1 were lysed under denaturing conditions and immunoprecipitated with an anti-ERK1/2 antibody. Proteins were analyzed by immunoblotting using the indicated antibodies. (H) Conceptual illustration showing the role of BAP1 in regulating MSC migration.

Journal: BMB Reports

Article Title: BAP1 controls mesenchymal stem cell migration by inhibiting the ERK signaling pathway

doi: 10.5483/BMBRep.2023-0174

Figure Lengend Snippet: BAP1 binds to ERK1/2 and regulates its ubiquitination status in a deubiquitinase activity-dependent manner. (A, C, E) 293T cells transfected with the indicated plasmids were harvested, lysed, and immunoprecipitated with an anti-GFP antibody. Thereafter, proteins were analyzed by immunoblotting using anti-FLAG and -GFP antibodies. (B) MSCs were lysed and immunoprecipitated using an anti-BAP1 antibody. Endogenous proteins were analyzed by immunoblotting using the indicated antibodies. (D, F) 293T cells transfected with the indicated plasmids were harvested, lysed under denaturing conditions, and immunoprecipitated with an anti-GFP antibody. Protein levels were analyzed by immunoblotting using anti-ubiquitin-HRP, -GFP, and -FLAG antibodies. (G) MSCs transfected with 20 nM siNon or siBAP1 were lysed under denaturing conditions and immunoprecipitated with an anti-ERK1/2 antibody. Proteins were analyzed by immunoblotting using the indicated antibodies. (H) Conceptual illustration showing the role of BAP1 in regulating MSC migration.

Article Snippet: Furthermore, the BAP1 expression pattern observed in the Human Protein Atlas supports an inverse correlation between BAP1 expression and cell motility, with blood cells, known for their high motility, showing notably lower levels of BAP1 expression ( of the SI).

Techniques: Ubiquitin Proteomics, Activity Assay, Transfection, Immunoprecipitation, Western Blot, Migration